Name one type of special stain used to identify bacteria in tissue samples.

Prepare for the ASCP Histotechnician Certification Exam with practice questions and detailed explanations. Enhance your knowledge and skills to excel in the exam.

The Gram stain is specifically designed for identifying bacteria in tissue samples, making it an essential technique in histopathology and microbiology. This staining method differentiates bacteria into two main categories: Gram-positive and Gram-negative, based on the structure of their cell walls. During the procedure, crystal violet is used as the primary stain, followed by iodine as a mordant, and then a decolorizing agent. Finally, a counterstain such as safranin is applied. The final color of the bacteria helps in assessing their type, aiding in diagnosis and treatment planning.

Other staining methods listed, like hematoxylin, are primarily used for general tissue staining rather than for specific identification of bacteria. Alice stain is not widely recognized in the context of histopathology or microbiology, and Masson's trichrome stain serves to differentiate connective tissue and muscle fibers rather than identifying bacteria. Thus, the Gram stain stands out as the ideal method for this purpose.

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